Multiplex real-time PCR assay using TaqMan probes for the identification of Trypanosoma cruzi DTUs in biological and clinical samples

by Cura CI, Duffy T, Lucero RH, Bisio M, Péneau J, Jimenez-Coello M, Calabuig E,  Gimenez MJ, Valencia Ayala E, Kjos SA, Santalla J, Mahaney SM, Cayo NM, Nagel C,  Barcán L, Málaga Machaca ES, Acosta Viana KY, Brutus L, Ocampo SB, Aznar C, Cuba Cuba CA, Gürtler RE, Ramsey JM, Ribeiro I, VandeBerg JL, Yadon ZE, Osuna A,  Schijman AG. PLOS Neglected Tropical Diseases 2015, doi: 10.1371/journal.pntd.0003765.

Summary: The genetic diversity of Trypanosoma cruzi parasites, which causes Chagas disease, is related to geographical distribution and transmission cycles, and might play a role in clinical manifestations as well as in anti-parasitic chemotherapy response. A reproducible genotyping strategy was developed that proved useful to differentiate between the six Discrete Typing Units (DTUs) of T.cruzi in cultured stocks, vector and reservoir specimens, as well as in patients´samples, especially in those from individuals with acute, congenital infection or Chagas reactivation. It is amenable to quantification and automation for kit production.

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