A DBS method for quantitation of the new oral trypanocidal drug fexinidazole and its active metabolites

by Filali-Ansary A, Augé C, Abgrall A, Souchaud M, Pellissier F, Valverde Mordt O, Blesson S, Sanderink JG. Bioanalysis 2016, doi: 10.4155/bio-2016-0110.

Summary: Fexinidazole (FEX) is a nitroimidazole being developed as a new trypanocide treatment for human African trypanosomiasis/sleeping sickness. A specific, accurate and reproducible assay was developed for quantitation of FEX and its metabolites M1 and M2 in dried blood spots (DBS), collected via finger-prick from healthy subjects. DBS samples were stable for 24 h at 37°C with 95% relative humidity, and 58 weeks desiccated at room temperature, making DBS finger-prick sampling a simple, practical method for determining FEX, M1 and M2 concentrations in clinical studies in Africa.

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