Validation and clinical evaluation of a novel method to measure miltefosine in leishmaniasis patients using dried blood spot sample collection

by Kip AE, Rosing H, Hillebrand MJ, Blesson S, Mengesha B, Diro E, Hailu A, Schellens JH, Beijnen JH, Dorlo TP. Antimicrobial Agents and Chemotherapy. 2016 Jan 19. pii: AAC.02976-15.

Summary: To facilitate future pharmacokinetic studies of combination treatments against leishmaniasis in remote endemic regions, a simple and cheap sampling methodology was required for miltefosine quantification. The aim of this study was to validate a liquid chromatography-tandem mass spectrometry method to quantify miltefosine in dried blood spots, and to validate its use in Ethiopian visceral leishmaniasis patients.  The method was demonstrated to be a valid and practical alternative to testing blood sampled from veins, and can be applied in future miltefosine pharmacokinetic studies in leishmaniasis patients.

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