Cytokines and chemokines measured in dried SLA-stimulated whole blood spots for asymptomatic Leishmania infantum and Leishmania donovani infection.

by Ibarra-Meneses AV, Mondal D, Alvar J, Moreno J, Carrillo E. Scientific Reports 2017, 7(1):17266, doi 10.1038/s41598-017-17315-z.

Summary: Whole blood stimulation with soluble Leishmania antigen (SLA), followed by plasma cytokine and chemokine determination, provides a means of detecting subjects with asymptomatic Leishmania infection. Protein Saver 903 cards were found to allow for the transport of SLA-stimulated samples by courier at ambient temperature, with CXCL10 and CXCL9 providing robust markers for identifying asymptomatic subjects from two endemic areas. This easy procedure opens up new possibilities for field studies in resource limited settings, which could help in Leishmania control.

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